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Fig. 4 | Journal of Neuroinflammation

Fig. 4

From: Fluoxetine inhibited the activation of A1 reactive astrocyte in a mouse model of major depressive disorder through astrocytic 5-HT2BR/β-arrestin2 pathway

Fig. 4

Astrocytic 5-HT2BR knockdown blocks effects of fluoxetine on CMS-induced depressive-like behaviors and A1 astrocyte reactivity. A Sucrose preference percentage of mice during 10 week treatment after AAV injection for 4 weeks. B Immobility time of mice in TST and FST. C, D Expression of C3 on astrocyte in the hippocampus DG of CON and CMS mice after AAV injection. E, F Protein levels of C3 in the hippocampus. G The levels of A1-special genes in the hippocampus. Scar bar, 20 μm in C. Quantitative data are mean ± s.e. Data were analyzed using two-way ANOVA, then combined with Tukey’s multiple comparison to assess the differences between groups. A, B: n = 9 CON with Control AAV and saline; n = 8 (B), n = 9 (A) CON with Control AAV and FLX; n = 8 (B-FST), n = 10 (A, B-TST) CMS with Control AAV and saline; n = 8 (B), n = 10 (A) CMS with Control AAV and FLX; n = 8 CON with mHT2BR AAV and saline; n = 8 CON with mHT2BR AAV and FLX; n = 8 (B-TST), n = 9 (A, B-FST) CMS with mHT2BR AAV and saline; n = 8 CMS with mHT2BR AAV and FLX; CG: n = 4 per group; biologically independent animals. A: ***P < 0.001 vs respective Control mice treated with saline; ###P < 0.001 vs CMS mice injected with Control AAV and treated with saline; $$P < 0.01vs CMS mice injected with Control AAV and treated with fluoxetine; B, D, F and G: *P < 0.05, **P < 0.01, and ***P < 0.001 vs respective Control mice treated with saline; #P < 0.05, ##P < 0.01, and ###P < 0.001 vs CMS mice injected with Control AAV and treated with saline; $P < 0.05, and $$$P < 0.001

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