Fig. 2

Extract, culture, and sorting of BMSCs, and identification and label of BMSC-exos. A Bone-marrow primary adherent cells before sorting (P0, Primary cells; P1, passage 1; P2, passage 2; P3, passage 3). B CD44⁺/CD45⁻ (96.4%) and CD11b⁻ (99.2%) BMSCs were sorted out by flow sorting, BMSCs grew into fibrous and colony-like growth, scale bar 100 μm. C Exosome morphology under transmission electron microscope. D Western blot photos of exosome markers. E Fluorescence imaging of exosome labeled with PKH26