Fig. 4
From: Vagus nerve stimulation modulates hippocampal inflammation caused by continuous stress in rats
aVNS upregulates the expression of α7 nAChR and ChAT in the hippocampus of CS animals. A RT-PCR analysis of α7 nAChR mRNA expression in the hippocampus. Upper images of agarose gel electrophoresis are the representatives of four independent experiments. Quantification of DNA band intensity relative to actin is plotted in lower panel. B Western blot analysis of α7 nAChR in the hippocampal tissues. C Immunofluorescence images of α7 nAChR signals in the granule cell layer (GCL) of the dentate gyrus (DG) and CA3 and CA1 pyramidal cell layers. The nuclei of neurons were visualized by staining with Hoechst 33258 (blue). D Western blot analysis of ChAT protein in the hippocampal tissue. In B and D, upper images are the representatives from four independent experiments. Quantification of band intensity relative to actin is plotted (lower panel). **p < 0.01, ***p < 0.001 (One-way ANOVA)