Table 1 Summary of antibody details

Anti-tyrosine-hydroxylase (host: rabbit, polyclonal)

Abcam

ab112

[65]

AB_297840

Full length protein from rat pheochromocytoma

1:2000

Anti-tyrosine-hydroxylase (host: mouse, monoclonal)

Sigma

T2928

[66]

AB_477569

rat tyrosine-hydroxylase

1:2000

Anti-UCN1

(host: goat, polyclonal)

Santa Cruz

SC1825

[21]

AB_2304014

C terminus peptide fragment of rat UCN1

1:200

Anti-UCN1

(host: rabbit, polyclonal)

Salk Institute

Prof. WW. Vale

PBL#5779

[67]

AB_2315527

rat UCN1 (amino acids 1–40)

1:20,000

Anti-GFAP (host: rabbit, monoclonal)

Abcam

Ab33922

[68]

AB_732571

C terminus synthetic peptide (sequence commercially sensitive)

1:1500

Anti-GFAP

(mouse monoclonal)

Novocastra

NCLLGFAP-GA5

[69]

AB_563739

Porcine spinal cord

1:1000

Anti-IBA1

(host: rabbit, polyclonal)

Wako Ltd

019–19,741

[70]

AB_839504

C terminus synthetic peptide of IBA1

1:1000

Anti-IBA1 (host: goat, polyclonal)

Wako Ltd

011–27,991

[71]

Synthetic peptide corresponding to C-terminal of IBA1

1:500

Anti-alpha-synuclein

(host: mouse, monoclonal)

Abcam

Ab1903

[72]

AB_302665

Recombinant full length protein corresponding to human alpha-synuclein

1:2000

Anti-NeuN (host: mouse, monoclonal, (clone A60)

Millipore

MAB377

[73]

AB_2298772

Purified cell nuclei from mouse brain

1:1000

Anti-5-HT

(host: mouse, monoclonal)

Universite´ Claude Bernard, Lyon, France

Dr. Luciene Leger

Custom made

[18]

BSA-conjugated 5HT

1:20,000

Anti-CD68

(Host: rabbit, polyclonal)

Abcam

ab125212

[74]

AB_10975465

Synthetic peptide AFCITRRRQSTYQPL

1:100

Anti-caspase 3 (host: rabbit, polyclonal)

Abcam

ab49822

[75]

AB_868673

aa167-175 within the p17 subunit (cleaved caspase)

1:4000

Anti-TNF-alpha (host: mouse, monoclonal)

Abcam

ab220210

[74]

AB_2892586

Recombinant full length protein corresponding to human TNFalpha

1:200

Anti-iNOS

(host: rabbit, monoclonal)

Abcam

ab178945

[76]

AB_2861417

Recombinant fragment (information is proprietary to the supplier)

1:250

Normal Donkey Serum

Jackson Immunoresearch

017–000-121

AB_2337258

-

2%

Alexa Fluor 488 donkey anti-mouse IgG (H + L)

Jackson Immunoresearch

706–545-148

AB_2340472

-

1:400

Alexa Fluor 488 donkey anti-goat IgG (H + L)

Jackson Immunoresearch

706–545-148

AB_2340472

-

1:600

Cy3 donkey Anti-Rabbit IgG (H + L)

Jackson Immunoresearch

711–165-152

AB_2307443

-

1:800

Alexa Fluor 647 donkey Anti-Rabbit IgG (H + L)

Jackson Immunoresearch

711–605-152

AB_2492288

-

1:500

Alexa Fluor 647 donkey Anti-Goat IgG (H + L)

Jackson Immunoresearch

705–605-003

AB_2340436

-

1:500

Biotin-SP (long spacer) donkey anti-rabbit IgG (H + L)

Jackson Immunoresearch

711-065-152

AB_2340593

-

1:200