Fig. 16

Knockdown Gpx4 in the Hip antagonized the antidepressant and anxiolytic effects of EDA. A Schematic timeline of the experimental procedure. B Fluorescence image of a fixed brain section that expressed AAV2/8-CMV–EGFP–miRNAi(Gpx4) in the Hip. Scale bar, 500 μm. C Social interaction test. D Sucrose preference test. E Open field test. F Elevated plus maze test. G Tail suspension test. H Forced swimming test. I Novel object recognition test. All the data are expressed as mean ± SEM (n = 10 per group). ***p < 0.001 versus the CON group. ^#p < 0.05, ^##p < 0.01, ^###p < 0.001 versus the CSDS group. ^&p < 0.05, ^&&p < 0.01 versus the CSDS + EDA group