Table 1 Primer sequences used in the qRT-PCR
From: Edaravone ameliorates depressive and anxiety-like behaviors via Sirt1/Nrf2/HO-1/Gpx4 pathway
Gene | Primer sequences |
|---|---|
Sirt1 | Forward: 5′-GCTGACGACTTCGACGACG-3′ Reverse: 5′-TCGGTCAACAGGAGGTTGTCT-3′ |
Nrf2 | Forward: 5′-TCTTGGAGTAAGTCGAGAAGTGT-3′ Reverse: 5′-GTTGAAACTGAGCGAAAAAGGC-3′ |
HO-1 | Forward: 5′-AAGCCGAGAATGCTGAGTTCA-3′ Reverse: 5′-GCCGTGTAGATATGGTACAAGGA-3′ |
Gpx4 | Forward: 5′-CTGCTCTTCCAGAGGTCCTG-3′ Reverse: 5′-GAGGTGTCCACCAGAGAAGC-3′ |
β-Actin | Forward: 5′-ACTGCCGCATCCTCTTCCT-3′ Reverse: 5′-TCAACGTCACACTTCATGATGGA-3′ |