Fig. 5

Running exercise upregulated microglial GLUT5 but not neuronal GLUT3 in APP/PS1 mice. a Representative immunofluorescence staining of GLUT5 (red), IBA1 (green), and DAPI (blue) in the DG, CA1 and CA3 regions of WT_Sed, AD_Sed, WT_Run, and AD_Run mice. Scale bar: 25 μm. b Quantification results of the ratio of GLUT5⁺/IBA1⁺ microglia in the hippocampus of WT_Sed, AD_Sed, WT_Run, and AD_Run mice. c GLUT5 and GLUT3 expression in the hippocampus of WT_Sed, AD_Sed, WT_Run, and AD_Run mice is shown by western blotting. d, e Quantification results of GLUT5/β-tubulin and GLUT3/β-tubulin. n = 3. Data are shown as the mean ± SD, *p < 0.05, **p < 0.01, ***p < 0.001