Fig. 6

Running exercise maintained TREM2 levels and reduced sTREM2 levels in APP/PS1 mice. a Relative expression of several top DEGs among WT_Sed mice, AD_Sed mice, and AD_Run mice. b Representative z-stack images (0.22 μm steps) of TREM2 (red) and IBA1 (white) microglia in the DG of WT_Sed, AD_Sed, WT_Run, and AD_Run mice and TREM2-positive puncta outside IBA1 were masked. Scale bar: 25 μm. c, d Levels of TREM2 and sTREM2 in the hippocampus of WT_Sed, AD_Sed, WT_Run, and AD_Run mice were determined by ELISA. n = 7–9. e The level of sTREM2 in the plasma of WT_Sed, AD_Sed, WT_Run, and AD_Run mice was determined by ELISA. n = 10. f Pearson’s correlation between plasma sTREM2 levels and hippocampal FDG uptake. g The expression of SPP1, TYROBP, ADAM10, p-SYK (Tyr525/526), and SYK in the hippocampus of WT_Sed, AD_Sed, WT_Run, and AD_Run mice was detected by western blotting. h–k Quantification results of SPP1/β-tubulin, TYROBP/β-tubulin, ADAM10/β-actin, and p-SYK/SYK. n = 3. Data are shown as the mean ± SD, *p < 0.05, **p < 0.01, ***p < 0.001