Fig. 5

Effects of MT5-MMP on basal synaptic transmission in cortical primary neurons. A Microphotograph of a representative pyramidal shaped neuron (arrow) in a 11–14 DIV culture. Scale bar: 25 μm. B Histograms of membrane capacitance (pF; pico Farad) and C input resistance (MΩ: mega Ohm) of WT, MT5^−/−, Tg, and TgMT5^−/− recorded pyramidal neurons. Black bars represent control (untreated) conditions (n = 13, 8, 11 and 10 neurons for each genotype, respectively). Grey bars represent IL-1β treatment 10 ng/mL for 24 h (n = 11, 10, 13, and 16 neurons for each genotype, respectively). D Representative traces of miniature global post-synaptic currents (gPSCs) obtained for a voltage clamped at − 50 mV in control (left) and IL-1β-treated conditions (right) (pA: pico Ampere; ms: millisecond). E and F Histograms showing the mean values of gPSCs amplitude (pA), frequency (Hz: Hertz). Black bars represent control (untreated) conditions (n = 12, 8, 10 and 7 neurons for each genotype, respectively). Grey bars represent IL-1β treatment 10 ng/mL for 24 h (n = 8, 10, 10, 12 neurons for each genotype, respectively). Values for B, C and E, F are the mean ± SEM of recorded neurons from 3 independent cultures. *p < 0.05, **p < 0.01 and ***p < 0.001 between untreated and treated cultures in the same genotype; ^#p < 0.05, ^##p < 0.01 and ^###p < 0.001 between genotypes. ANOVA followed by post hoc Fisher’s LSD test