Fig. 6

Effects of MT5-MMP deficiency and IL-1β on APP metabolism in cortical neural cell cultures. A Immunoblot analyses of soluble APP (sAPP) and canonical full length APP (APPfl) detected with the 22C11 antibody in primary cultures treated or not with IL-1β (10 ng/mL) and/or DAPT (10 μM), and the corresponding β-actin normalized quantifications. B Immunoblot analyses of APP CTF fragments detected with APP-CTF antibody in primary cultures treated or not with IL-1β (10 ng/mL) and/or DAPT (10 μM), and the corresponding β-actin normalized quantifications. AAV-C99 (right) indicates a positive control. WT cells were infected for 5 days with AAV-C99 and recovered at 11 DIV. Note that only C83 levels were detectable with DAPT treatment. C and D Measurements by MSD multiplex assay of Aβ40 and Aβ42 levels (pg/mL) in primary cultures in control (black) and IL-1β (grey) conditions. Values are the mean ± SEM of 8–16 for A, B and 4–5 for C, D independent cultures by genotype. *p < 0.05 and ***p < 0.001 between untreated and treated cultures with IL-1β and DAPT in the same genotype; ^#p < 0.05, ^##p < 0.01 between genotypes. ANOVA followed by post hoc Fisher’s LSD test. IB immunoblot, O.D. optical density, A.U. arbitrary units