Fig. 8

Effects of MT5-MMP deficiency on C99 overexpression in cortical neural cell cultures. A Immunoblot analyses of C99 and C83 detected with the APP-CTF antibody in primary cortical cultures after transduction with AAV-empty or AAV-C99 (2 μL at 5.10¹² vg/mL), treated or not with DAPT (10 μM), and the corresponding β-actin normalized quantifications for C99 (B) and C83 (C). D Measurement of human Aβ40 levels (pg/mL) in primary cortical cultures using the ThermoFisher Scientific ELISA kit. Values are the mean ± SEM of 3–5 independent cultures by genotype. **p < 0.01 and ***p < 0.001 between untreated and treated cultures with DAPT in the same genotype; ^#p < 0.05, ^##p < 0.01 and ^###p < 0.001 between genotypes. ANOVA followed by post hoc Fisher’s LSD test. IB immunoblot, O.D. optical density, A.U. arbitrary units