Skip to main content
Fig. 1 | Journal of Neuroinflammation

Fig. 1

From: Lowering blood cholesterol does not affect neuroinflammation in experimental autoimmune encephalomyelitis

Fig. 1

Hypercholesterolemia induced by LDLr deficiency do not exacerbate EAE disease. A Serum level of total cholesterol (tChol) in female LDLr−/− (mean ± SD; n = 6) and wild-type mice (mean ± SD; n = 12 mice). B EAE in female wild-type and LDLr−/− mice. The course of EAE is shown as clinical score (mean ± SEM; n = 8 mice). C EAE in male wild-type and LDLr−/− mice. The course of EAE is shown as clinical score (mean ± SEM; n = 9 mice). The female and male EAE experiments were not performed at the same time. Data are representative of three experiments. D Histopathological stainings and quantifications of spinal cord sections of non-immunized WT (WT NI) or immunized WT and LDLr−/− mice at day 16 post-immunization for cellular infiltration (H&E). Five sections per mouse were quantified (n = 3 mice). Scale bars 500 µm (top panels), 100 µm (bottom panels). E Flow cytometry analysis of the total proportion (%) of the leukocyte (viable CD45+), lymphocyte T (CD3+) and lymphocyte T CD4+ (CD4+) in the CNS 14 days after EAE immunization (mean ± SD; n = 3–5 mice). p < 0.0001; NS, not significant; p values were determined by unpaired Student’s t test (A and D), a Mann–Whitney U test (B, C) and a two-way ANOVA with Sidak’s post hoc test (E).

Back to article page