Fig. 4

Anti-PCSK9 treatment does not show altered systemic immune responses. In vitro restimulation of splenocytes isolated from EAE immunized anti-PCSK9 treated mice versus control group with different concentrations of MOG_35-55 peptide or CON A. A Proliferative response was determined by [3H]-thymidine integration and expressed in counts per minute (CPM) (mean ± SEM, n = 4 mice). B–D Cytokine production was determined by ELISA: Secretion of IL-2 (B), IL-17A (C) and IFN-γ (D) were measured by ELISA after 48 h of culture with the indicated concentration of MOG_35–55 (mean ± SEM, n = 3–4 mice). E–G Flow cytometric analysis of the frequencies of IL-17⁺RORγt⁺ (E), IL-17⁺IFNγ⁺ (F) and IFN-γ⁺Tbet⁺ (G) expression in CD3⁺CD4⁺CD44⁺ T cells at day 6 after restimulation with indicated concentration of MOG_35–55. Results shown are from mice pooled from two independent experiments (mean ± SD, n = 8 mice). Data are representative of three experiments.∗∗∗p < 0.001; p values were determined by two-way ANOVA with Sidak’s post hoc test (A–G)