Fig. 3

Co-administered l-DOPA is able to block IL-1β production by primary human microglia in response to canonical (a), non-canonical (b), and α-syn-mediated (c) NLRP3 inflammasome activation. 2 experiments, n = 3. d The protective effect of l-DOPA is reversed by the AADC inhibitor carbidopa, indicating that l-DOPA-mediated inhibition of IL-1β production is a result of the conversion of l-DOPA to DA. e DRD1 agonism with SKF82958 completely blocks IL-1β production by canonical NLRP3 inflammasome activation, similar to DA and l-DOPA, while DRD2 agonism with quinpirole has no effect. 2 experiments, n = 3. f DRD1 and DRD2 agonism both partially block IL-1β production induced by α-syn, suggesting a shared role for these receptors in α-syn-mediated NLRP3 inflammasome activation. Representative experiment. ****p < 0.0001 by two-way ANOVA with Bonferroni multiple testing correction. Shapes represent individual experiments