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Fig. 3 | Journal of Neuroinflammation

Fig. 3

From: The role of S100B/RAGE-enhanced ADAM17 activation in endothelial glycocalyx shedding after traumatic brain injury

Fig. 3

Astrocyte TBI model was characterised by elevated S100B, RAGE. A Morphology of primary cultured rat astrocytes identified with positive staining of GFAP. DAPI was used as a nuclear marker. Scale bar = 100 μm. B Morphological changes of astrocytes before and after the equiaxial stretch injury induced by Flexcell® FX-5000™ Tension System, × 100. C Cell viability of astrocytes at different timepoints after stretch injury detected by CCK-8. *p < 0.05 compared with the Control group, n = 4. D Representative blots of S100B and RAGE in astrocytes, S100B and sRAGE in cultured medium after stretch injury or exogenous S100B administration. And quantification (histograms of right panels) of S100B, RAGE in cell lysates and S100B, sRAGE in medium from representative blots shown in left panel. GADPH was used as a soluble loading control for cellular protein content. The content of S100B and sRAGE in cultured medium was shown directly by gray scale. *p < 0.05 compared with Control group, n = 3

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