Fig. 5

Activation of S100B/RAGE signal mediates EG damage. A, B Representative blots of syndecan-1 in injured brain and in serum of TBI rats, and TBI plus ONO-2506 (A) or FPS-ZM-1 (B). GADPH was used as a soluble loading control (left panel). In addition, quantification (histograms of right panel) of syndecan-1 in brain lysates and serum from representative blots shown in left panel. *p < 0.05 compared with Sham group, ^#p < 0.05 compared with TBI group, n = 6. C Representative blots of Syndecan-1 in RAECs and in cultured medium after stretch injured-conditioned medium or exogenous S100B administration. GADPH was used as a soluble loading control (left panel). In addition, quantification (histograms of right panels) of Syndecan-1 from representative blots shown in left panel. *p < 0.05 compared with Control group, n = 3. D, E Representative blots of syndecan-1 in RAECs and in cultured medium after treatment of stretch injured-conditioned medium or injured medium plus S100B inhibitor ONO-2506 (E) or FPS-ZM-1 (F). GADPH was used as a soluble loading control (left panel). In addition, quantification (histograms of right panel) of syndecan-1 in cell lysates and medium from representative blots shown in left panel. *p < 0.05 compared with control group, ^#p < 0.05 compared with conditioned medium-treated group, n = 3. F Representative confocal images showing the subcellular localization of syndecan-1 and vWF in the brain tissue of rats treated with TBI, TBI plus ONO-2506 or FPS-ZM-1. Scale bar = 100 μm. The immunofluorescence intensity of syndecan-1 in brain tissue from five fields per sample in each group. The relative fluorescence intensity was quantified and reported as relative fluorescence units (RFUs). *p < 0.05 compared with Sham group, ^#p < 0.05 compared with TBI group, n = 3. G Representative confocal images showing the expression and distribution of syndecan-1 in RAECs treated with ONO-2506 or FPS-ZM-1. Scale bar = 50 μm. The immunofluorescence intensity of syndecan-1 in cells from five fields per sample in each group was quantified and reported as relative fluorescence units (RFUs). *p < 0.05 compared with control group, ^#p < 0.05 compared with conditioned medium-treated group, n = 3