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Fig. 9 | Journal of Neuroinflammation

Fig. 9

From: The role of S100B/RAGE-enhanced ADAM17 activation in endothelial glycocalyx shedding after traumatic brain injury

Fig. 9

S100B/RAGE mediated activation of ADAM17 promotes secondary brain and lung injury after TBI. A, D Representative H&E staining images showing the histology of cortex of injured-side brain tissue (A) or lungs (D) of rats 6 h after the onset of TBI in TBI, and TBI plus S100B inhibitor ONO-2506, RAGE inhibitor FPS-ZM-1, or ADAM17 inhibitor TAPI-1 group, (×100). B, E Quantification of water content of brain (B) or lungs (E) from rats of TBI and TBI plus respective inhibitor. *p < 0.05 compared with Sham group; #p < 0.05 compared with TBI group, n = 6. C, F Gross observation of brains (C) and lungs (F) with Evans blue indicating the vascular leakage of tissue in rats of TBI and TBI plus respective inhibitor. In addition, quantification of Evans blue concentration in tissue from rats of TBI, and TBI plus respective inhibitor (far right histogram). *p < 0.05 compared with Sham group; #p < 0.05 compared with TBI group, n = 6. G Representative confocal images showing the subcellular localization of syndecan-1 and vWF in lung tissue from rats 6 h after the onset of TBI, and TBI plus S100B inhibitor ONO-2506, RAGE inhibitor FPS-ZM-1, or ADAM17 inhibitor TAPI-1 group. Scale bar = 100 μm. The immunofluorescence intensity of syndecan-1 in lung tissue from five fields per rat in each group. The relative fluorescence intensity was quantified and reported as relative fluorescence units (RFUs). *p < 0.05 compared with Sham group, #p < 0.05 compared with TBI group, n = 3

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