Fig. 2

Characterization of RBC-EVs from A53T mice, WT mice and SNCA KO mice. A Western blot to assess the presence of EV markers Alix and TSG101 in RBC-EV lysates and RBC lysates from A53T mice, WT mice and SNCA KO mice. B Nanoparticle trafficking to analyze the size distribution and concentration of RBC-EVs from A53T mice, WT mice and SNCA KO mice. C Representative electron micrograph images of RBC-EVs from A53T mice, WT mice and SNCA KO mice. Scale bar, 500 nm. D Levels of total α-syn in RBC-EVs from A53T mice, WT mice and SNCA KO mice. E Levels of oligomeric α-syn in RBC-EVs from A53T mice, WT mice and SNCA KO mice. Values are means ± S.E.M, one-way ANOVA test. *, P < 0.05; **, P < 0.01; ***, P < 0.001; ****, P < 0.0001