Fig. 3

α-Syn-containing RBC-EVs from A53T mice induced immune sensitization of THP-1 cells. A Quantitative analysis of IL1b, IL6 and TNF mRNA levels using qPCR, in THP-1 cells pretreated with RBC-EVs from A53T mice, WT mice or SNCA KO mice at resting state (blue) and stimulated with LPS for 24 h (red). B Quantitative analysis of pro-inflammatory cytokines IL-1β, IL-6, TNF-α, IL-8, IFN-γ, IL-2, and IL-12p70, and C anti-inflammatory cytokines IL-4, IL-10, and IL-13 using MSD, released by THP-1 cells pretreated with RBC-EVs from A53T mice, WT mice or SNCA KO mice at resting state (blue) and stimulated with LPS for 24 h (red). N = 3 independent experiments in each group. Values are means ± S.E.M, two-way ANOVA with Bonferroni’s post hoc test. *, P < 0.05; **, P < 0.01; ***, P < 0.001; ****, P < 0.0001