Fig. 6

Increased LRRK2 in hyperactivated monocytes induced by A53T RBC-EVs. A Western blot to assess the level of LRRK2 and p-Rab10 in monocytes of A53T mice and WT mice. B Quantitative analysis of the level of LRRK2 and ratio of p-Rab10 to Rab10 in monocytes of A53T mice and WT mice. C Western blot to assess the level of LRRK2 in THP-1 cells treated with RBC-EVs from A53T mice and WT mice. D Quantitative analysis of the level of LRRK2 in THP-1 cells treated with RBC-EVs from A53T mice and WT mice. E Western blot to assess the level of Rab10 in THP-1 cells treated with RBC-EVs from A53T mice and WT mice. F Quantitative analysis of the level of p-Rab10 in THP-1 cells treated with RBC-EVs from A53T mice and WT mice. G Quantitative analysis of LRRK2 mRNA level in THP-1 cells treated with RBC-EVs from A53T mice and WT mice. H Quantitative analysis of IL1b, IL6 and TNF mRNA levels using qPCR, in THP-1 cells pretreated with 100 nM Mli2 at resting state (blue) and stimulated with LPS (red). I Quantitative analysis of pro-inflammatory cytokines IL-1β, IL-6, TNF-α, IL-8, IFN-γ, IL-2, and IL-12p70, and J anti-inflammatory cytokines IL-4, IL-10, and IL-13 using MSD, released by THP-1 cells pretreated with 100 nM Mli2 at resting state (blue) and stimulated with LPS for 24 h (red). N = 3 independent experiments in each group. Values are means ± S.E.M, t test (B), one-way ANOVA test (D, F, G), two-way ANOVA with Bonferroni’s post hoc test (H–J). *, P < 0.05; **, P < 0.01; ***, P < 0.001; ****, P < 0.0001