Fig. 2

Sox2 overexpression protected HCs from aminoglycoside-induced injury in the neonatal stage A Experimental protocol for C-D. Cochlear epithelia from Atoh1CreER^+/–/Sox2OE^+/– and control mice that received tamoxifen injection at P0–P1 were dissected and cultured at P2. Cochlear explants were treated with 1 mM neomycin for 6 h and harvested after an additional 24 h. B The scheme for cell counting. C Representative images of Myosin7a⁺ cells in Atoh1CreER^+/–/Sox2OE^+/– and control mice treated with or without neomycin. D Quantification of the cochlear HCs per 100 µm in the Atoh1CreER^+/–/Sox2OE^+/– and control groups. Data are presented as means ± SEM; n = 6 for each group; ***P < 0.001. Scale bar = 20 µm