Fig. 8

Sox2 overexpression suppressed LPS-induced upregulation of IL6 and TNF-α. A IL6 immunofluorescence in cochlear cross-section after LPS treatment. Myosin7a is used as a marker of cochlear HCs. (B) Western blot analysis of sensory epithelia shows increased expression levels of TNF-α and IL6 in control mice after LPS treatment, but the LPS-induced increase of the TNF-α and IL6 protein was significantly inhibited in Prestin-Sox2OE mice. β-Actin served as the sample loading control. Data are presented as means ± SEM.; n = 3. *P < 0.05, **P < 0.01. Scale bar = 20 μm