Fig. 3

M-cone photopic ERG response is decreased in the active phase of bMOG EAE. A, B M- and S-cone-mediated photopic ERG responses were, respectively, recorded after flash stimulation at 504 and 365 nm. Representative M-cone-dependent photopic waves showed clear b-wave amplitude reduction at 504-nm stimulation (A). Representative S-cone-dependent photopic waves also suggest moderate decrease in retinal activity at 365-nm stimulation (B). C Quantitative analyses of luminance-response curves revealed strong diminution of M-cone b-wave amplitudes in EAE mice (n = 18) compared with CFA (n = 11) or naive (n = 13) mice. Peak times were not affected by EAE. D S-cone-mediated ERG response was only significantly reduced in EAE eyes relative to naive mice, but did not statistically change when compared to CFA-treated mice. Peak times were similar between the groups. E, F The amplitude and peak time of b-waves induced by M- and S-cone stimulation did not differ between EAE (n = 16) and control treatments (CFA, n = 16; naive, n = 5) on day 32. Statistics: two-way ANOVA, Tukey post hoc test; *: P < 0.05, **: P < 0.01, ***: P < 0.001, ****: P < 0.0001. Green stars indicate statistical significance between EAE and CFA-treated mice. Blue stars indicate statistical significance between EAE and naive mice