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Fig. 5 | Journal of Neuroinflammation

Fig. 5

From: B cell-dependent EAE induces visual deficits in the mouse with similarities to human autoimmune demyelinating diseases

Fig. 5

bMOG induces retinal gliosis. A Quantification of glial Gfap, Vim and neuroprotection markers (Edn2, Fgf2) in retinae by RT-qPCR revealing increased gliosis in EAE. The four markers were significantly higher in EAE (n = 7) than in naive (n = 6) and CFA (n = 5) groups (one-way ANOVA, Tukey post hoc test, *: P < 0.05, **: P < 0.01, NS = not significant). BE Qualitatively, on EAE retinal cryosections, the GFAP signal was stronger in GS+ radial Müller cell extensions in comparison to naive mice. At higher magnifications (be), the spread of GFAP was prominent in Müller cell endfeet and in the inner retina. F Quantification of Cd68 in retinal lysates by RT-qPCR suggesting an increased activation of microglia/macrophage in EAE mice (one-way ANOVA, **: P < 0.01). G Qualitatively, immunofluorescent staining showed higher expression of CD68 in microglial cells identified using the Iba1 marker (red) in EAE retinae. At higher magnification, the dotted distribution of CD68 in microglial cells suggests increased lysosomal activity associated with microglial phagocytosis and activation. Scale bars: B = 100 µm, b = 50 µm, G = 100 µm, close-up in G = 25 µm. ONL outer nuclear layer, OPL outer plexiform layer, INL inner nuclear layer, IPL inner plexiform layer, GCL ganglion cell layer

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