Table 1 Antibodies used for immunofluorescence (IF) and western blotting (WB)
Name | Species | Dilution IF | Dilution WB | Source |
|---|---|---|---|---|
Olig2 | Rabbit | 1:500 | Millipore | |
FluoroMyelin | N/A | 1:300 | Life Technologies | |
RBPMS | Guinea pig | 1:500 | PhosphoSolutions | |
M-opsin | Rabbit | 1:500 | 1:5000 | Millipore |
S-opsin | Goat | 1:200 | 1:2000 | Santa Cruz |
Peanut agglutinin | N/A | 1:250 | Vector Laboratories | |
GAPDH | Mouse | 1:20,000 | Abcam | |
GFAP | Rabbit | 1:500 | Dako | |
GS | Mouse | 1:1000 | Millipore | |
Iba-1 | Goat | 1:500 | Novus Biologicals | |
CD68 | Rat | 1:1000 | Abcam | |
Syt2 | Mouse | 1:200 | Abcam | |
ChAT | Goat | 1:100 | Millipore | |
NF-H | Mouse | 1:500 | Millipore | |
Melanopsin | Rabbit | 1:2500 | Advanced Targeting Systems | |
B3T | Mouse | 1:1000 | Promega | |
P-Erk1/2 | Rabbit | 1:100 | Cell Signaling |