Fig. 4

IgE-IC induces acute ocular itch in a FcεRIα-dependent manner. A, B Eye-towards wiping (A) and scratching (B) response of FcεRIα^+/+ and FcεRIα^−/− mice as ocular application of capsaicin (Cap). No difference was detected for FcεRIα^+/+ and FcεRIα^−/− mice for both reaction to Vehicle 1 and Cap. n = 7, Student’s t-test. C, D Eye-towards wiping (C) and scratching (D) response of FcεRIα^+/+ and FcεRIα^−/− mice as ocular application of histamine (His). No difference was detected for FcεRIα^+/+ and FcεRIα^−/− mice for both reaction to Vehicle 2 and His. n = 7, t test. E, F Eye-towards scratching (E) and wiping (F) response of FcεRIα^+/+ and FcεRIα^−/− mice as ocular instillation of IgE-IC or PBS. ^##P < 0.01, ^###P < 0.001, IgE-IC vs. PBS; ***P < 0.001, FcεRIα^+/+ vs. FcεRIα^−/−; Two-way ANOVA followed by Bonferroni’s post hoc test comparisons. G, H Eye-towards scratching (G) and wiping (H) response of c-Kit^+/+ and c-Kit ^W-sh/W-sh mice as ocular instillation of IgE-IC. n = 7, ^#P < 0.05, ^###P < 0.001, ^####P < 0.0001, vs. Baseline, two-way ANOVA followed by Bonferroni’s post hoc test comparisons