Fig. 6
Representative images of retinal explant cryosections cocultured with 1.104 MSCs at DEV 0 and DEV 7 immunolabeled with Iba1 (red) (A), CD68 (red) (B) and DiO-labeled MSCs (green) at ×200 magnification (scale bar = 100 µm). C Quantification of Iba1 immunoreactivity (expressed as Raw Integrated Density). n = 4–6 animals/group. Ordinary one-way ANOVA was performed. *P < 0.05. D Quantification of CD68 immunoreactivity (expressed as Raw Integrated Density). n = 4–6 animals/group. Ordinary one-way ANOVA was performed. E, F RT-qPCR analysis of ITGAM and CD68 expression in retinal explants at DEV 0 and DEV 7. mRNA levels are presented after normalization with the housekeeping gene Rps18. n = 12–13 animals/group. Unpaired t-test was performed for unpaired comparisons. ****P < 0.0001