Table 1 Antibodies used for the MSC characterization using flow cytometry
Antibody | Host | Supplier | Reference |
|---|---|---|---|
Positive markers | |||
CD29 | Armenian hamster | eBioscience | 11-0291-80 |
CD73 | Mouse | BD Biosciences | 551123 |
CD90 | Mouse | eBioscience | 45-0900-80 |
Negative markers | |||
CD11b/c | Rabbit | BD Pharmingen | 554862 |
CD45 | Mouse | Serotec | MCA43PE |
CD68 | Mouse | Serotec | MCA341R |
Ia | Mouse | Bio-Rad | MCA46G |
Isotype control FITC | Armenian hamster | eBioscience | 11-4888-81 |
IgG2a kappa Isotype control | Mouse | eBioscience | 45-4724-80 |
IgG1, κ | Mouse | BD Pharmingen | 554680 |
IgG-UNLB | Mouse | SouthernBiotech | 0107-01 |