Fig. 1
Increased Aβ levels and Aβ plaques in microglial VPS35 deficient 5XFAD brain. a–d Increased Aβ plaque depositions in 3-mo VPS35CX3CR1:5XFAD male (a, b) and female (c, d) brains. High-magnification images in cortex (CTX), hippocampus (HIP), subiculum (SUB), and thalamus (TH), marked by dashed squares, were shown. Quantification data, the area covered by Thio-S+ Aβ plaques, were presented in b, d (n = 9–10 per group, mean ± SD, *P < 0.05, **P < 0.01, Student’s t test), Scale bar = 600 µm. e–h Increased insoluble human Aβ40 and Aβ42 levels in CTX and HIP as well as soluble human Aβ42 in CTX of VPS35CX3CR1:5XFAD mice, as compared with those of Ctrl:5XFAD mice (n = 3 male per group, mean ± SD, **P < 0.01, Student’s t test)