Fig. 4
Enhanced cognitive dysfunction, but not depression, in young adult microglial VPS35 deficient 5XFAD mice. a–c Open field test (OFT). Representative traces of mouse movement path in Ctrl:5XFAD mice and VPS35CX3CR1:5XFAD mice (3-MO) (a). Total distance traveled (b) and time spent inner zone (c) were shown (n = 6 male mice per group, mean ± SD). d–f Immobility time in forced swimming test (FST), tail suspension test (TST), and sucrose preference by sucrose preference test (SPT) were shown. The sucrose preference = consumed sucrose/total consumed liquid. (n = 6 male mice per group, mean ± SD). g–i Y-maze test. Schematic diagram (g), and reduced spontaneous alternations, but no the number of arm entries, in VPS35CX3CR1:5XFAD mice were shown (n = 6 male mice per group, mean ± SD, **P < 0.01). J–m Morris water maze (MWM) assay. Ctrl:5XFAD (n = 6) and VPS35CX3CR1:5XFAD (n = 6) male mice were examined. The VPS35CX3CR1:5XFAD mice exhibited increases in the latency and travel distance to the hidden platform (mean ± SD, *P < 0.05). n–o Motion trajectory in Ctrl:5XFAD mice and VPS35CX3CR1:5XFAD mice after removing hidden platform at day 6 (n), and quantification of the time spend in the hidden platform zone (o). (Mean ± SD, **P < 0.01, Student’s t test)