Fig. 5
Reduced plaque-associated myeloid cells in microglial VPS35 deficient 5XFAD brain. a, b Representative images for GFP (green) (a), Iba1 (b), and Thio-S (white) immunostaining in the CTX (cortex). High-magnification images, marked by dashed squares, were shown in right panels. The plaque associated microglia were defined by number of microglia in a plaque centered circle within 50 μm in diameter (marked by dashed red circles). Scale bars, 10 μm. c–f Quantification analyses of data in a, b. The plaque associated microglia (number per plaque) by co-immunostaining GFP or Iba1 with Thio-S were shown in c; the microglial density (number per unit area) (319.45 μm2) was shown in d; the microglia-Aβ association by assessing the percentage of covered area was shown in e; and the soma volume of Aβ associated microglia measured by Imaris software was shown in f (n = 5–6 male mice per group, mean ± SD, **P < 0.01, Student’s t test). g Representative images for Iba1 (green) and Thio-S (white) immunostaining in the HIP. High-magnification images were shown in right panels. Scale bar, 10 μm. h–i Quantitative analysis of data in g. The plaque associated microglia (number per plaque) (h) and microglial density (number per unit area) (i) were shown (n = 6 male mice per group, mean ± SD, **P < 0.01, Student’s t test)