Fig. 6

Decreased DAM in microglial VPS35 deficient 5XFAD brain. a Schematic diagram of Aβ associated microglia or DAM (disease associated microglia), and markers for DAM; and summary of the results about DAMs in control and microglial VPS35 deficient 5XFAD brain. b, c Representative images for Iba1 (green), LPL (red), and Thio-S (gray) immunostaining (b), and quantification of relative LPL fluorescence intensity in Aβ associated and Aβ un-associated microglia (c). d, e Representative images for Iba1 (green), Trem2 (red) and Thio-S (gray) immunostaining (d), and quantification of relative Trem2 fluorescence intensity in Aβ associated and Aβ un-associated microglia (e). f, g Representative images for Iba1 (green), Clec7a (red) and Thio-S (gray) immunostaining (f), and quantification of relative Clec7a fluorescence intensity in Aβ associated and Aβ un-associated microglia (g). Scale bars, 10 μm. Data in c, e, and g are presented as mean ± SD (n = 5 male mice per group, *P < 0.05, **P < 0.01, N.S., P > 0.05, Student’s t test)