Fig. 3
Microglia depletion increases HSV-1 lethality and tissue viral loads of TMLeD/LeD mice. A The numbers of microglia in mice treated without (Control) or with PLX5622 right before infection are shown. B The survival rates of TMLeD/LeD mice fed with control chow (Control; n = 7) or chow containing PLX5622 (PLX5622; n = 8) and infected with HSV-1 are shown. C Virus titers in the indicated mouse tissues are shown. Leukocytes purified from brains of infected mice at 5 dpi were assayed for markers of D microglia (CD45intCD11b+), E macrophages (CD45hiCD11b+Ly6G−), F neutrophils (CD45hiCD11b+Ly6G+), G T cells (CD45hiCD3+), H activated microglia (CD45intMHC-II+CD11b+), I activated myeloid cells (CD45+MHC-II+), or J activated macrophage (CD45hiMHC-II+CD11b+Ly6G−) by flow cytometry. The data represent means ± or + SEM (error bars) of 5–8 samples per data point in panel C and 4–6 samples per group in panels A and D–J. *P < 0.05; **P < 0.01; and ***P < 0.001