Fig. 5

TM-LeD knockdown enhances the microglia phagocytic activity and capacity to reduce HSV-1 replication in neurons. A WT or TM^+/LeD HMC3 cells were mock-infected (–) or infected (+) with virus for 24 h (MOI = 1) and assayed for CD68 and β-actin by Western blotting. The levels of CD68 normalized to β-actin are shown. The mean of group with mock-infected (–) WT microglia only was set as 100%. B WT or TM^+/LeD HMC3 cells were infected with HSV-1 (MOI = 1) for 24 h and subjected to the zymosan phagocytosis assay. C SK-N-SH cells were co-cultured without or with WT or TM^+/LeD HMC3 cells, infected with HSV-1 (MOI = 0.001), and harvested at the indicated hours for viral titration. The data represent means ± or + SEM (error bars) of 3–7 samples per group or data point. *P < 0.05 and **P < 0.01