Fig. 3

Inflammasome assembly and mature IL-1β release in the spinal cord after sciatic nerve axotomy. a Speck-like costaining of NLRP3 and ASC in injured neurons 3 days after the injury (arrows). b Representative WB images of mature IL-1β and β-actin proteins 3 days after unilateral axotomy of sciatic nerve. c Quantification of active IL-1β protein expression. N = 3 animals/group. The graph shows values normalized to β-actin levels and relative to contralateral side. Mean values are shown on bars. Bars represent average ± SEM. **p < 0.01 (ANOVA with Bonferroni post hoc, ax. + vehicle vs. contralateral side). ^#p < 0.05, ^##p < 0.01 (ANOVA with Bonferroni post hoc, compared to ax. + vehicle). Ax. axotomy