Fig. 5

NLRP3 upregulation in glial cells 7 days after peripheral nerve axotomy. a, b Colocalization of NLRP3 with the microglial marker Iba1 (solid arrows) and with astrocytes labeled with GFAP (dashed arrows) in the injured ventral horn 7 days after sciatic nerve axotomy in vehicle- a and MCC950-treated animals (b). c Quantification of NLRP3-Iba1 colocalization. Results represent the ratio of colocalizing pixels, relative to microglial area. N = 4 animals/group, **p < 0.01 (Student’s paired t-probe). d Quantification of NLRP3-GFAP colocalization. Graphs indicate the ratio of colocalizing pixels, relative to astroglial area. N = 4 animals/group, n.s. non-significant (Student’s paired t-probe). Mean values are presented on all bars. Bars represent average ± SEM. Ax. axotomy