Table 1 Number of animals used in different experimental setups
Methods | Experiments | Subgroups/treatments | Number of animals used |
|---|---|---|---|
qPCR | Time-dependent changes in the expression of inflammasome-related genes | Intact | 3 |
Sham | 3 | ||
6 h after axotomy | 3 | ||
1 day after axotomy | 3 | ||
3 days after axotomy | 3 | ||
7 days after axotomy | 3 | ||
21 days after axotomy | 3 | ||
Effect of NLRP3 and P2X4 inhibition | Intact | 3 | |
1 day after ax. + vehicle | 3 | ||
1 day after ax. + 5-BDBD | 3 | ||
1 day after ax. + MCC950 | 3 | ||
ISH | Cellular localization of IL1B mRNA | 1 day after axotomy | 3 |
1 day after intraspinal injection of LPS + MDP (positive control) | 1 | ||
IF | Colocalization of inflammasome components | 1 day after axotomy | 3 |
3 days after axotomy | 3 | ||
7 days after axotomy | 3 | ||
Quantification NLRP3 and Iba1/GFAP colocalization | 7 days after ax. + vehicle | 3 | |
7 days after ax. + MCC950 | 3 | ||
Quantification of axonal regeneration | 5 days after ax./coapt. + vehicle | 3 | |
5 days after ax./coapt. + MCC950 | 3 | ||
5 days after ax./coapt. + intrathecal goat IgG | 4 | ||
5 days after ax./coapt. + intrathecal IL-1β neutralization | 4 | ||
WB | Effect of NLRP3 and P2X4 inhibition | 3 days after ax. + vehicle | 3 |
3 days after ax. + 5-BDBD | 3 | ||
3 days after ax. + MCC950 | 3 | ||
IHC | Quantification of microglia and astroglia activation | 3 days after axotomy | 4 |
3 days after ax. + vehicle | 4 | ||
3 days after ax. + MCC950 | 4 | ||
3 days after ax. + intrathecal goat IgG | 4 | ||
3 days after ax. + intrathecal IL-1β neutralization | 4 | ||
7 days after axotomy | 4 | ||
7 days after ax. + vehicle | 4 | ||
7 days after ax. + MCC950 | 4 | ||
7 days after ax. + intrathecal goat IgG | 4 | ||
7 days after ax. + intrathecal IL-1β neutralization | 4 | ||
SFI and retrograde labeling | Quantification of sciatic nerve regeneration | Axotomy and coaptation | 5 |
Ax./coapt. + vehicle | 5 | ||
Ax./coapt. + 5-BDBD | 5 | ||
Ax./coapt. + MCC950 | 5 | ||
Total number of animals | 135 | ||