Table 2 Primers used for qPCR
Genes | Forward primers (5’ → 3’) | Reverse primers (5’ → 3’) |
|---|---|---|
IL1B | TGCCACCTTTTGACAGTGATG | TGATGTGCTGCTGCGAGATT |
CASP1 | GGGACCCTCAAGTTTTGCC | GACGTGTACGAGTGGTTGTATT |
NLRP3 | GGCGAGACCTCTGGGAAAAA | CTTCAAGGCTGTCCTCCTGG |
NLRP6 | AGCTGAGAACGCTGTGTCG | AACTTGGGAACCCCGAAGC |
AIM2 | GGTGGCGTCAGGAAGTTTTC | GCCGGTCAACAACAGCATTT |
P2X4 | AAAGGTGTGGCTGTGACCAA | TCCAGTCCCAATTCCACTGC |
GAPDH | GTGAAGGTCGGTGTCAACG | GTGAAGACGCCAGTAGACTC |