Fig. 3

Transcription and translation levels of C3 and S100A10 after erythrocyte lysate stimulation in primary astrocytes. A Protein expression of C3 and S100A10 in primary astrocytes after erythrocyte lysate stimulation [C3: F (3, 8) = 74.4, P < 0.0001; S100A10: F (3, 8) = 519.8, P < 0.0001]. The blots are representative of other replicates in those groups. B mRNA level of C3 [F (3, 8) = 272.9, P < 0.0001). C mRNA level of S100A10 [F (3, 8) = 293, P < 0.0001). D expression of TNF-α [F (3, 8) = 37.69, P < 0.0001]. E expression of IL-1β [F (3, 8) = 20.16, P = 0.0004]. The data were analyzed using one-way analysis of variance and all data are expressed as the mean ± standard deviation. *P < 0.05 represents a statistically significant difference between the two groups. Each experiment was repeated three times