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Fig. 4 | Journal of Neuroinflammation

Fig. 4

From: Homer1 promotes the conversion of A1 astrocytes to A2 astrocytes and improves the recovery of transgenic mice after intracerebral hemorrhage

Fig. 4

Effects of Homer1-OE and Homer1-KD on transcription and translation level of C3 and S100A10 in vivo and in vitro. A Effects of Homer1-OE and Homer1-KD on cell activity. the modified cells were replaced with ACM medium and cultured for 48 h to reach the most serious state of inflammation [normal medium: F (2, 6) = 1.760, P = 0.2504; ACM medium: F (2, 6) = 0.6375, P = 0.5610]. qPCR was used to detect the transcription levels of Homer1 (B) [F (3, 8) = 146.5, P < 0.0001], C3 (C) [F (3, 8) = 179.6, P < 0.0001) and S100A10 (D) [F (3, 8) = 196.7, P < 0.0001] in each group; WB was used to detect the translation level of Homer1, C3 and S100A10 (E) in each group and quantify the results (F) [Homer1: F (3, 8) = 334.0, P < 0.0001; C3: F (3, 8) = 136.7, P < 0.0001; S100A10: F (3, 8) = 146.1, P < 0.0001]; The blots are representative of other replicates in those groups. ELISA was used to detect the secretion levels of TNF-α (G) [F (3, 8) = 56.76, P < 0.0001] and IL-1β (H) [F (3, 8) = 140.1, P < 0.0001] in cell supernatant of each group. In vivo, WB was used to detect the translation levels of Homer1, C3 and S100A10 in the brain tissue of mice in each group on the 3rd day after ICH (I), and the results were quantified (J) [Homer1: F (3, 16) = 103.3, P < 0.0001; C3: F (3, 16) = 170.8, P < 0.0001; S100A10: F (3, 16) = 106.2, P < 0.0001]; The blots are representative of other replicates in those groups. qPCR was used to detect the transcriptional levels of Homer1 (K) [F (3, 16) = 157.3, P < 0.0001], C3 (L) [F (3, 16) = 389.2, P < 0.0001] and S100A10 (M) [F (3, 16) = 581.7, P < 0.0001] in the brain of mice 3 days after ICH. The data were analyzed using one-way analysis of variance and all data are expressed as the mean ± standard deviation. *P < 0.05 represents a statistically significant difference between the two groups. ns: no statistical difference. Each experiment was repeated three times

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