Fig. 7

HCQ administration attenuated BBB disruption at 3 day post-TBI. a Representative 3D reconstructed images of the colocalization of CD31 (red) and Iba1 (green). b Representative images of brain tissue and EB leakage at 3 day post-TBI. c Quantification of EB extravasation. n = 5 per group. Data are presented as the mean ± SD. ****p < 0.0001 vs. sham group, ^##p < 0.01, vs. TBI + Vehicle group. d–f Representative photographs showing immunofluorescence staining and the quantitative analysis of ZO-1 and claudin-5 expression in the lesioned boundary at 3 day postinjury. n = 5 per group. The data are presented as the mean ± SD. *p < 0.05, **p < 0.01 vs. sham group, ^#p < 0.05 vs. TBI + Vehicle group. ns: no significant difference. g–i Representative western blot bands and densitometric quantification of ZO-1 and claudin-5 expression. n = 4 per group. The data are presented as the mean ± SD. *p < 0.05, **p < 0.01 vs. sham group, ^#p < 0.05 vs. TBI + Vehicle group. ns: no significant difference