Fig. 3

Anti-integrins block the rolling and adhering of leukocytes on cerebral endothelial cells during colitis. Intravital microscopy was used to identify rolling and adhering leukocytes in colitic female mice treated with an anti-integrin or an isotype control 18–22 h prior to imaging. A Anti-α4 integrin significantly reduced rolling (t = 2.9, df 14, *P = 0.01, n = 8 mice/group) and adhering (t = 4.3, df 14, ***P < 0.001, n = 8 mice/group) leukocytes in colitic mice compared to isotype-treated colitic controls. B Representative images from intravital microscopy. CD31 was used to label cerebral endothelial cells (blue), Rho6G was used to label leukocytes (red). C Anti-α4ß7 significantly reduced rolling leukocytes in colitic mice (t = 2.9, df 12, *P = 0.01, n = 6–8 mice/group) but not adhering (t = 2.0, df 12, P = 0.07, n = 6–8 mice/group) compared to isotype-treated colitic controls. D Representative images from intravital microscopy. CD31 was used to label cerebral endothelial cells (blue), Rho6G was used to label leukocytes (red). E Anti-MAdCAM-1 significantly reduced rolling leukocytes in colitic mice (t = 2.4, df 9, *P = 0.04, n = 5–6 mice/group) but not adhering (t = 1.8, df 9, P = 0.11, n = 5–6 mice/group) compared to isotype-treated colitic controls. F Representative images from intravital microscopy. CD31 was used to label cerebral endothelial cells (blue), Rho6G was used to label leukocytes (red). Scale bar for B, D, F: 25 µm