Fig. 5

Blocking neutrophils reduces the rolling and adhering of leukocytes on cerebral endothelial cells during colitis. Intravital microscopy was used to visualize the rolling and adhering of leukocytes in colitic female mice treated with anti-Ly6G to block neutrophils compared to isotype controls. A Anti-Ly6G significantly reduced both rolling (t = 4.0, df 11, **P < 0.01; n = 5–8 mice/group) and adhering (t = 2.3, df 11, *P = 0.04; n = 5–8 mice/group) leukocytes in colitic mice compared to isotype-treated colitic controls. B Representative images from intravital microscopy. CD31 was used to label cerebral endothelial cells (blue), Rho6G was used to label leukocytes (red). Scale bar: 25 µm