Fig. 6

Anti-Ly6C depletion of monocytes reduces the rolling and adhering of neutrophils on cerebral endothelial cells during colitis. Intravital microscopy was used to visualize the rolling and adhering of neutrophils and monocytes in colitic female mice treated with anti-Ly6C to block monocytes or treated with an isotype control. A Colitic mice treated with anti-Ly6C significantly reduced rolling (t = 8.5, df 8, ***P < 0.001; n = 4–6 mice/group) but not adhering (t = 0.1, df 8, P = 0.89; n = 4–6 mice/group) monocytes (Ly6C positive cells) compared to colitic mice treated with an isotype control. B Anti-Ly6C treatment that reduces monocytes, significantly reduced rolling (t = 3.3, df 9, **P = 0.009; n = 5–6 mice/group) and adhering (t = 2.7, df 9, *P = 0.02, n = 5–6 mice/group) neutrophils in colitic mice compared to colitic mice treated with an isotype control. C Representative images from intravital microscopy. Ly6C was used to label monocytes (blue), Ly6G was used to label neutrophils (red). Scale bar: 25 µm