Fig. 7
In vivo neutralization of α4β7 integrin modulates colitis-induced increases in cortical cytokine levels. To address the possible role of the α4β7 integrin in initiating neuroimmune activation, cortical cytokine levels of colitic female mice were assessed after anti-α4β7 treatment. Increased cortical cytokines levels of A IL-1β and B CCL2 induced by colitis were significantly reduced by anti-4β7 integrin antibody treatment (F(2, 15) = 4.79, *P = 0.02 and F(2, 15) = 5.08, *P = 0.02, respectively; colitis + anti-α4β7 vs. all other groups, one-way ANOVA; n = 6 mice per group). C. α4β7 integrin neutralization did not alter colitis-induced changes in cortical IL-10 levels (F(2, 13) = 6.45, *P = 0.01; colitis + anti-α4β7 and colitis + isotype vs. control group, one-way ANOVA; n = 6 mice per group)