Skip to main content
Fig. 7 | Journal of Neuroinflammation

Fig. 7

From: Macrophage elastase (MMP12) critically contributes to the development of subretinal fibrosis

Fig. 7

The effect of MMP408 on macrophage accumulation in subretinal fibrosis. A Cryosections from subretinal fibrosis eyes were stained for collagen-1 (red) and F4/80 (green) and imaged by confocal microscopy. B Magnified area of box in (A) showing F4/80+MMP12+ cells (arrows) inside and around the subretinal fibrotic lesion. C The percentage of F4/80+Collagen-1+ cells among all F4/80+ macrophages. D–H Representative confocal images of RPE/choroid flatmounts stained for collagen-1 (red) and F4/80 (green) from control non-treatment group (D), vehicle (DMSO) group (E) and MMP408 group (F). Scale bar = 50 µm. G Magnified area of box in (E) showing F4/80+collagen-1+ cells (arrows) in the fibrotic lesion. H Quantitative analysis of the total F4/80+ cells in RPE-choroid flatmount in different groups. Mean ± SD, n = 6–9 eyes from 4–5 mice, *p < 0.05, **p < 0.01. Kruskal–Wallis with Dunn’s multiple comparisons test

Back to article page