Fig. 1

Evaluation of brain nerve function of C57BL/6 mice before and after Angiostrongylus cantonensis infection by behavioral tests. Different behavioral tests were used to evaluate the neurological changes of C57BL/6 mice at different timepoints after infection. a Morris water maze was divided into acquisition test (spatial learning) on the first 4 days and the probe test (spatial memory) on the 5th day. b In the acquisition test phase of Morris water maze, the time for mice to reach the platform decreased with the increase of the number of learning days. After 14 days and 21 days of infection, the spatial learning ability of C57BL/6 mice was significantly reduced (*P < 0.05, ***P < 0.001). c In the probe test stage of Morris water maze, the time that C57BL/6 mice stayed in the target quadrant decreased with the increase in the number of days of infection, indicating that the spatial memory ability of the mice was affected by the infection, and significantly decreased after 14 days and 21 days of infection (*P < 0.05, ***P < 0.001). d NOR task was used to detect new things recognition memory in mice. C57BL/6 mice in the 14-day and 21-day infection groups cannot distinguish new and old things, and their ability to recognize new things and memory was significantly reduced (*P < 0.05, *P < 0.05). Y maze was used to evaluate the spatial working memory of mice. e Total number of times the mice entered each arm was recorded. The total number of entries decreased significantly after 14 days and 21 days after infection, indicating that the mice's desire to explore after infection was reduced (***P < 0.001, ***P < 0.001). f There was no significant difference in working memory ability between different groups of mice before/after infection. The data were analyzed by One-Way ANOVA and Two-Way ANOVA