Fig. 1

Expression profile of irisin and integrin αVβ5 after ICH. A Representative western blot bands of time course and quantitative analyses of irisin and integrin αVβ5 expression in the ipsilateral hemisphere after ICH. *p < 0.05, **p < 0.01, ***p < 0.001 vs. sham group. Error bars are represented as mean ± SD. n = 6 per group. B Comparison of plasma irisin levels between Sham and ICH mice at 6 h and 24 h after ICH surgery. *p < 0.05, **p < 0.01, ***p < 0.001 vs. sham group, n = 6 per group. C Schematic illustration of brain tissue showing the area in the perihematomal region (indicated by white box) from where the images were taken for immunofluorescence staining. D Representative images of colocalization of integrin αVβ5 (red) with microglia/macrophage (Iba-1, green), astrocytes (GFAP, green) and neurons (NeuN, green) in the sham group and the perihematomal area of ICH (24 h) group. Nuclei were stained with DAPI (blue). Scale bar = 50 μm, n = 2/group. E Triple-label immunofluorescence confocal microscopy for microglia/macrophage marker Iba-1 (magenta), integrin αVβ5 (red), and irisin (green) in the perihematomal region at 24 h after ICH. Rectangle: cell enlarged and three-dimensional (3D)-rendered by Imaris 9.2 (Bitplane, Switzerland) in the down panel. Nuclei were stained with DAPI (blue). Scale bar of the 3D-rendered cell = 10 μm