Fig. 4

Effects of irisin post-treatment on neuronal apoptosis and neuronal apoptotic molecular markers after ICH. A, B Representative images of the co-localization of TUNEL (green) with neurons (NeuN, red) and FJC (green) staining in the perihematomal area at 24 h after ICH. C Brain sample with schematic illustration showing the area (indicated by the white square) used for TUNEL and FJC-positive cell counting in the perihematomal region. D Quantitative analyses of TUNEL and FJC-positive cells in the perihematomal area at 24 h after ICH (n = 6/group). E, F Representative western blot bands and quantitative analyses of Bax and Bcl-2 protein levels at 24 h after ICH. *p < 0.05, **p < 0.01, ***p < 0.001 vs. sham group; ^#p < 0.05, ^##p < 0.01 vs. ICH + vehicle group, mean ± SD, one-way ANOVA, Tukey test, n = 6/group, scale bar = 50 μm