Fig. 1

Microglia in the brain of GFAP-IL6 versus GFAP-IFN mice have unique turnover patterns. a–c Representative immunofluorescence images (Iba1⁺ microglia, green; BrdU⁺, red; DAPI, blue) from the cerebellum of 1-month-old WT (a), GFAP-IL6 (b) and GFAP-IFN (c) mice. d–f Representative images (Iba1⁺ microglia, green; TUNEL⁺, red; DAPI, blue) from the hippocampus of 6-month-old WT (d), GFAP-IL6 (e) and GFAP-IFN (f) mice. Scale bars, 20 μm. g–o Quantification of the total number of Iba1⁺ microglia per mm² (g-i), the number of Iba1⁺BrdU⁺ microglia per mm² (j–l) and the number of Iba1⁺TUNEL⁺ apoptotic microglia per section (m–o) in the cerebellum (g, j, m), cortex (h, k, n) and hippocampus (i, l, o) at 1, 3 and 6 months of age. n = 3–5 mice/group. Graphs show individual values per mouse and mean ± SEM. *, p < 0.05 compared with WT of the same age; ^, p < 0.05 compared with GFAP-IL6 of the same age; #, p < 0.05 compared with 1-month-old of the same genotype; x, p < 0.05 compared with 3-month-old of the same genotype using two-way ANOVA with Tukey’s post-test